deoxyribonucleoside triphosphate

Fox RM, Piddington SK, Tripp EH, Tattersall MH. The information on this site is not to be used for diagnosing or treating any health concerns you may have - please contact your physician or health care professional for all your medical needs. Deoxyribonucleoside triphosphate pools in human bone marrow and leukaemic cells.  |  Deoxyribonucleoside triphosphate accumulation by leukemic cells. deoxyadenosine triphosphate, dATP; deoxythymidine triphosphate, dTTP; deoxycytosine triphosphate, dCTP; and deoxyguanosine triphosphate, dGTP) are usually added to PCR and RT-PCR mixes in equimolar amounts, though if the target DNA sequence to be PCR amplified comprises mainly dATP/dTTP (AT … Kim W, Le TM, Wei L, Poddar S, Bazzy J, Wang X, Uong NT, Abt ER, Capri JR, Austin WR, Van Valkenburgh JS, Steele D, Gipson RM, Slavik R, Cabebe AE, Taechariyakul T, Yaghoubi SS, Lee JT, Sadeghi S, Lavie A, Faull KF, Witte ON, Donahue TR, Phelps ME, Herschman HR, Herrmann K, Czernin J, Radu CG. In vitro incubations of leukemic cells with deoxyribonucleosides may provide a rational basis for the use of these compounds as chemotherapeutic agents. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. J Clin Invest.

Tattersall MH, Ganeshaguru K, Hoffbrand AV. Last Update: %%BUILD_DATE%%. Evaluation of ribonucleoside and deoxyribonucleoside triphosphate pools in cultured leukemia cells during exposure to methotrexate or methotrexate plus thymidine. Get the latest research from NIH: https://www.nih.gov/coronavirus. The toxicity of the deoxyribonucleosides, 2'-deoxyadenosine, 2'-deoxyguanosine, and thymidine, for human T lymphoblasts is mediated by the accumulation of the corresponding deoxyribonucleoside triphosphate (dATP, dGTP, or dTTP, respectively). Take our user survey and have your say: By using this site you agree to our Terms of Use. Peterson AR, Heidelberger C, Benedict WF.  |  What is the best treatment for my condition. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. USA.gov. Deoxycoformycin in the treatment of mature T-cell leukaemias. Get the latest research from NIH: https://www.nih.gov/coronavirus.

Our servers have detected that you are accessing this site from a country that is a member of the European Union. Evidence for abnormal translational control of protein synthesis. A deoxynucleoside triphosphate is a nucleotide containing 3 phosphate groups attached to the 5' carbon of the deoxyribose sugar of the nucleoside.There are four different deoxynucleoside triphosphates; deoxyadenosine-5' triphosphate (dATP), deoxyguanosine-5' triphosphate (dGTP), deoxythymidine-5' triphosphate (dTTP), deoxycytidine-5' triphosphate (dCTP)  |  Basic Life Sci. NLM J Clin Invest. These medical condition or symptom topics may be relevant to medical information for Deoxyribonucleoside triphosphate: In PHA-stimulated lymphocytes, the antifolate drugs methotrexate, pyrimethamine and trimethoprim, all produced a fall in dTTP and a rise in dATP concentrations within 1 h. These effects could be reversed by folinic acid. 6-Mercaptopurine caused a fall in dATP and dGTP, the fall in dATP being marked after 4 h incubation.

PHA-stimulation of lymphocytes caused a marked increase of the deoxyribonucleoside triphosphate concentrations, particularly of dTTP, between 24 and 48 h of culture. 1991 Nov;64(5):903-6. doi: 10.1038/bjc.1991.423.  |  Chottiner EG, Cloft HJ, Tartaglia AP, Mitchell BS. DATP is a purine 2'-deoxyribonucleoside 5'-triphosphate having adenine as the nucleobase. 2015 Sep;10(3):396-401. doi: 10.1007/s11481-015-9624-y.

Deoxyadenosine triphosphate as a mediator of deoxyguanosine toxicity in cultured T lymphoblasts. Br J Cancer. Information provided on this site is for informational purposes only; it is not intended as a substitute for advice from your own medical team. 1982 Jul 15;206(1):131-8. doi: 10.1042/bj2060131.

J Clin Invest. This page is not available in your area. Johnson CH, Patti GJ, Courade JP, Shriver LP, Hoang LT, Manchester M, Siuzdak G. J Neuroimmune Pharmacol.  |  Ecto-adenosine triphosphatase deficiency in cultured human T and null leukemic lymphocytes. HHS Clipboard, Search History, and several other advanced features are temporarily unavailable.

The deoxyribonucleoside triphosphate concentrations were increased approximately threefold in the nucleated marrow cells from patients with leukaemia and myeloproliferative diseases. Using a sensitive enzymic assay, we have measured the concentrations (pools) of these compounds in human bone marrow cells and in lymphocytes. PHA-stimulation of lymphocytes caused a marked increase of the deoxyribonucleoside triphosphate concentrations, particularly of dTTP, between 24 and 48 h of culture. Home | Symptoms | Diseases | Diagnosis | Videos | Tools | Forum | About Us | Terms of Use | Privacy Policy | Site Map | Advertise. Biochem J. DNA replication and repair require each of the four canonical deoxyribonucleoside triphosphates (dNTPs). Epub 2015 Aug 2. Deoxyribonucleoside triphosphate metabolic process Antibodies Antibodies for proteins involved in deoxyribonucleoside triphosphate metabolic process pathways; according to their Panther/Gene Ontology Classification. The toxicity of the deoxyribonucleosides, 2'-deoxyadenosine, 2'-deoxyguanosine, and thymidine, for human T lymphoblasts is mediated by the accumulation of the corresponding deoxyribonucleoside triphosphate (dATP, dGTP, or dTTP, respectively). 1980;28:94-101. doi: 10.1159/000386065. It is concluded that measurement of the deoxyribonucleoside triphosphates in human cells provides a new method of studying DNA synthesis in human disease states and of analysing the action of antimetabolite drugs on normal and diseased cells.

Oncogenic transformation of C3H/10T1/2 Cl 8 mouse embryo fibroblasts by inhibitors of nucleotide metabolism.

1986 Nov;78(5):1261-9. doi: 10.1172/JCI112710. USA.gov.

The mean concentrations (pmol/10(6) cells) in normal human bone marrow cells were: deoxyadenosine triphosphate (dATP) 1.5; deoxyguanosine triphosphate (dGTP) 0.4; thymidine triphosphate (dTTP) 1.4 and deoxycytidine triphosphate (dCTP) 0.6; and in normal phytohaemagglutinin (PHA)-stimulated lymphocytes (72 h cultures); dATP 3.7; dGTP 1.9; dTTP 9.4 and dCTP 2.9. This content is not available in your region.www.rightdiagnosis.com. Deoxynucleotide-interconverting enzymes and the quantification of deoxynucleoside triphosphates in mammalian cells. 2016 Apr 12;113(15):4027-32. doi: 10.1073/pnas.1524212113.

BCNU caused a significant fall in dATP and dCTP. Dibutyryl cyclic 3', 5' adenosine monophosphate and theophylline had no consistent effect on the deoxyribonucleoside triphosphate concentrations. This site needs JavaScript to work properly.

Biochemical reactions, even those as complex as replicating the DNA genome of cells, follow the principle that the process is regulated by both the substrate concentration and by the enzymes that mediate the process. Treatment of four individuals with acute lymphoblastic leukemia with the adenosine deaminase inhibitor, 2'-deoxycoformycin, resulted in elevations in plasma deoxyadenosine concentrations and in increments in lymphoblast dATP levels that were similar to those measured in lymphoblasts cultured with deoxyadenosine and deoxycoformycin prior to treatment. We have examined whether leukemic cells of non-T-cell origin are capable of accumulating deoxyribonucleotides in culture and whether this capability correlates with the activities of purine metabolizing enzymes in these cells. 1981 Aug;68(2):544-52. doi: 10.1172/jci110286.
This site needs JavaScript to work properly. Proc Natl Acad Sci U S A. Epub 2016 Mar 28. Eur J Clin Pharmacol. It is a purine 2'-deoxyribonucleoside 5'-triphosphate and a 2'-deoxyadenosine 5'-phosphate.It is a conjugate acid of a dATP(3-). NIH 1987 Mar;79(3):1001-5. doi: 10.1172/JCI112866.

NIH 5-Fluorouracil caused a fall in dTTP and in dCTP but no consistent changes in dATP; hydroxyurea caused a fall in dATP with a rise in dTTP. Get the latest public health information from CDC: https://www.coronavirus.gov. Determination of the nucleoside triphosphate contents of eggs and oocytes of Xenopus laevis. Effect of proguanil and cycloguanil on human lymphocytes in vitro. NLM  |  Fuller SA, Hutton JJ, Meier J, Coleman MS. Biochem J. Clipboard, Search History, and several other advanced features are temporarily unavailable. It has a role as an Escherichia coli metabolite and a mouse metabolite. What do you think about the features of this website? HHS COVID-19 is an emerging, rapidly evolving situation.

1985;31:465-79. doi: 10.1007/978-1-4613-2449-2_30. 1 R23 CA30025/CA/NCI NIH HHS/United States, NCI CPTC Antibody Characterization Program. Antibiot Chemother (1971).

Please enable it to take advantage of the complete set of features! Deoxyribonucleoside triphosphate: Related Topics. Get the latest public health information from CDC: https://www.coronavirus.gov. [18F]CFA as a clinically translatable probe for PET imaging of deoxycytidine kinase activity. Please enable it to take advantage of the complete set of features!
Deoxynucleotide triphosphate synonyms, Deoxynucleotide triphosphate pronunciation, Deoxynucleotide triphosphate translation, English dictionary definition of Deoxynucleotide triphosphate. We have found that non-T, non-B acute lymphoblastic leukemia cells with low ecto-5'-nucleotidase and high adenosine deaminase activities increase their dATP pools by greater than tenfold when exposed to deoxyadenosine and an inhibitor of adenosine deaminase in culture. Cells from 2 of 9 patients with chronic lymphocytic leukemia and 4 of 11 patients with acute nonlymphoblastic leukemia achieved similar elevations in dATP, but there was no relationship between dATP accumulation and adenosine deaminase, purine nucleoside phosphorylase, or ecto-5'-nucleotidase activities. Deoxyribonucleoside triphosphate: glycosamine consisting of a base linked to a deoxyribose sugar esterified with triphosphate on its glycose moiety. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/.

National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. 1985;28(3):287-90. doi: 10.1007/BF00543325. Alterations in Spinal Cord Metabolism during Treatment of Neuropathic Pain. 1972 Apr;127(3):597-605. doi: 10.1042/bj1270597. Effect of methotrexate and 1-beta-D-arabinofuranosylcytosine on pools of deoxyribonucleoside triphosphates in L1210 ascites cells. A biochemical basis for thymidine sensitivity. Elevated adenosine deaminase activity and hereditary hemolytic anemia. Copyright © 2014 Health Grades Inc. All rights reserved. The deoxyribonucleoside triphosphate concentrations were increased approximately threefold in the nucleated marrow cells from patients with leukaemia and myeloproliferative diseases. Deoxyribonucleic acid synthesis requires adequate cellular concentrations of the four deoxyribonucleoside triphosphates. Please see our Terms of Use.

COVID-19 is an emerging, rapidly evolving situation. Source: CRISP. Mitchell BS, Edwards NL, Koller CA. The four individual deoxynucleotides which make up a DNA sequence (i.e. INTRODUCTION.

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